{"id":219,"date":"2019-04-19T13:04:00","date_gmt":"2019-04-19T11:04:00","guid":{"rendered":"https:\/\/biophysics.pmfst.unist.hr\/?p=219"},"modified":"2023-10-11T13:31:15","modified_gmt":"2023-10-11T11:31:15","slug":"javna-obrana-doktorskog-rada-tomislava-roncevica","status":"publish","type":"post","link":"https:\/\/biophysics.pmfst.unist.hr\/en\/javna-obrana-doktorskog-rada-tomislava-roncevica\/","title":{"rendered":"Javna obrana doktorskog rada Tomislava Ron\u010devi\u0107a"},"content":{"rendered":"\n

OBAVIJEST<\/strong><\/p>\n\n\n\n

Javna obrana doktorskog rada studenta poslijediplomskog sveu\u010dili\u0161nog studija BIOFIZIKA<\/p>\n\n\n\n

TOMISLAVA RON\u010cEVI\u0106A<\/strong><\/h2>\n\n\n\n

pod naslovom<\/p>\n\n\n\n

\u201cIDENTIFICATION, REDESIGN AND CHARACTERIZATION OF ANURAN ANTIMICROBIAL PEPTIDES\u201d<\/strong><\/h2>\n\n\n\n

(From targeted DNA sequencing and database analyses to peptide antibiotics)<\/em><\/p>\n\n\n\n

odr\u017eat \u0107e se u \u010detvrtak, 25. travnja 2019. u 10:00 sati<\/strong> na Prirodoslovno-matemati\u010dkom fakultetu u Splitu (amfiteatar A0-1), pred \u010dlanovima Stru\u010dnog povjerenstva:<\/p>\n\n\n\n

    \n
  1. doc. dr. sc. Ana Maravi\u0107 (Prirodoslovno-matemati\u010dki fakultet Sveu\u010dili\u0161ta u Split), predsjednica<\/li>\n\n\n\n
  2. izv. prof. dr. sc. Karl Lohner (Institut za molekularne bioznanosti Sveu\u010dili\u0161ta u Grazu), \u010dlan<\/li>\n\n\n\n
  3. izv. prof. dr. sc. Maria Luisa Mangoni (Fakultet farmacije i medicine Sapiensa Sveu\u010dili\u0161ta u Rimu), \u010dlan<\/li>\n\n\n\n
  4. doc. dr. sc. Larisa Zorani\u0107 (Prirodoslovno-matemati\u010dki fakultet Sveu\u010dili\u0161ta u Split), zamjenski \u010dlan.<\/li>\n<\/ol>\n\n\n\n

    Mentori<\/strong>: prof. dr. sc. Jasna Puizina (Prirodoslovno-matemati\u010dki fakultet Sveu\u010dili\u0161ta u Splitu)
    prof. dr. sc. Alessandro Tossi (Odjel za \u017eivotne znanosti Sveu\u010dili\u0161ta u Trstu).<\/p>\n\n\n\n

    Title:
    IDENTIFICATION, REDESIGN AND CHARACTERIZATION OF ANURAN ANTIMICROBIAL PEPTIDES<\/strong>
    (From targeted DNA sequencing and database analyses to peptide antibiotics)<\/em><\/p>\n\n\n\n

    Naslov:
    IDENTIFIKACIJA, REDIZAJN I KARAKTERIZACIJA ANTIMIKROBNIH PEPTIDA IZ \u017dABA (ANURA)<\/strong>
    (Od ciljanog sekvenciranja DNK i analiza baza podataka prema peptidnim antibioticima)<\/em><\/p>\n\n\n\n

    Abstract:<\/h3>\n\n\n\n

    Antimicrobial peptides (AMPs) are structurally diverse molecules naturally produced by most organisms, with direct antimicrobial activity against pathogens and often showing other immune-related properties. Anurans are a particularly rich source of these peptides, with ~2000 different sequences reported in Database of Anuran Defense Peptides (DADP). A novel approach for simultaneous multi-species peptide identification has been developed by exploiting the highly conserved signal peptide region to design family specific forward degenerate primers, using RNAseq data available in the Sequence Read Archive (SRA) database, and using them to selectively amplify AMP-coding transcripts from small amounts of frog skin tissue. Additionally, novel peptides have been redesigned with the help of molecular descriptors using the Mutator software that links biophysical properties of the peptides with their biological activity. This software has been trained on helical AMPs of anuran origin and may provide best results if natural peptides of anuran origin are used as input. In both cases, a number of the identified\/designed peptides have been tested for antibacterial activity, toxicity and mode of action. Some of these proved to be potent antimicrobials with limited toxicity towards host cells.<\/p>\n\n\n\n

    Sa\u017eetak:<\/h3>\n\n\n\n

    Antimikrobni peptidi (AMP) su strukturalno razli\u010dite molekule prirodno sintetizirane u ve\u0107ini organizmima, s direktnom antimikrobnom aktivnosti, dok \u010desto pokazuju i druga imunolo\u0161ki povezana svojstva. Vodozemci iz reda Anura su naro\u010dito bogat izvor takvih peptida s ~2000 razli\u010dite sekvence pohranjene u \u2018Database of Anuran Defense Peptides\u2019 (DADP). Razvijen je novi pristup za istovremenu identifikaciju peptida iz vi\u0161e razli\u010ditih vrsta oslanjaju\u0107i se na izrazito o\u010duvane signalne sekvence za dizajn specifi\u010dnih po\u010detnica i podatke RNAseq dostupne u \u2018Sequence Read Archive\u2019 (SRA), te ih koriste\u0107i kako bi se selektivno amplificirali transkripti koji kodiraju AMP iz malih koli\u010dina tkiva ko\u017ee \u017eaba. Tako\u0111er, novi peptidi su redizajnirani uz pomo\u0107 molekularnih deskriptora koriste\u0107i ra\u010dunalni program Mutator koji povezuje biofizikalna svojstva peptida s njihovom biolo\u0161kom aktivno\u0161\u0107u. Taj alat razvijen je koriste\u0107i set helikalnih AMP-a \u017eabljeg podrijetla, te bi trebao dati najbolje rezultate ako se unose upravo takvi prirodni peptidi. U oba slu\u010daja, odre\u0111eni broj identificiranih\/dizajniranih peptida je testiran naspram antibakterijske aktivnosti, toksi\u010dnosti i mehanizma djelovanja. Neki od tih peptida su se pokazali kao dobri antimikrobni kandidati s neznatnom toksi\u010dno\u0161\u0107u naspram stanica doma\u0107ina.<\/p>\n\n\n\n

    Obavijest \u2013 javna obrana doktorskog rada Tomislava Ron\u010devi\u0107a<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

    OBAVIJEST Javna obrana doktorskog rada studenta poslijediplomskog sveu\u010dili\u0161nog studija BIOFIZIKA TOMISLAVA RON\u010cEVI\u0106A pod naslovom \u201cIDENTIFICATION, REDESIGN AND CHARACTERIZATION OF ANURAN ANTIMICROBIAL PEPTIDES\u201d (From targeted DNA sequencing and database analyses to peptide antibiotics) odr\u017eat \u0107e se u \u010detvrtak, 25. travnja 2019. u 10:00 sati na Prirodoslovno-matemati\u010dkom fakultetu u Splitu (amfiteatar A0-1), pred \u010dlanovima Stru\u010dnog povjerenstva: Mentori:<\/p>\n","protected":false},"author":1,"featured_media":701,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[26,16],"tags":[],"class_list":["post-219","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-defences-of-doctoral-theses","category-news-and-updates"],"acf":[],"_links":{"self":[{"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/posts\/219","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/comments?post=219"}],"version-history":[{"count":2,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/posts\/219\/revisions"}],"predecessor-version":[{"id":728,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/posts\/219\/revisions\/728"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/media\/701"}],"wp:attachment":[{"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/media?parent=219"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/categories?post=219"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/tags?post=219"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}