{"id":352,"date":"2023-04-18T10:55:00","date_gmt":"2023-04-18T08:55:00","guid":{"rendered":"https:\/\/biophysics.pmfst.unist.hr\/?p=352"},"modified":"2023-11-10T14:22:52","modified_gmt":"2023-11-10T12:22:52","slug":"javna-obrana-doktorskog-rada-zvonimira-bobana-mag-phys-2","status":"publish","type":"post","link":"https:\/\/biophysics.pmfst.unist.hr\/en\/javna-obrana-doktorskog-rada-zvonimira-bobana-mag-phys-2\/","title":{"rendered":"Javna obrana doktorskog rada Zvonimira Bobana, mag. phys."},"content":{"rendered":"\n

OBAVIJEST<\/strong><\/p>\n\n\n\n

Javna obrana doktorskog rada studenta poslijediplomskog sveu\u010dili\u0161nog (doktorskog) studija BIOFIZIKA<\/p>\n\n\n\n

ZVONIMIRA BOBANA, mag. phys.<\/strong><\/h2>\n\n\n\n

pod naslovom<\/p>\n\n\n\n

\u201cOPTIMIZACIJA ELEKTROFORMACIJE DIVOVSKIH UNILAMELARNIH VEZIKULA ZA RAZLI\u010cITE LIPIDNE SMJESE UZ NAGLASAK NA VISOKE KONCENTRACIJE KOLESTEROLA<\/strong>\u201d<\/strong><\/h2>\n\n\n\n

odr\u017eat \u0107e se u \u010detvrtak, 27. travnja 2023., u 15:00 sati<\/strong> na Prirodoslovno-matemati\u010dkom fakultetu u Splitu (amfiteatar A1-2), pred \u010dlanovima Stru\u010dnog povjerenstva:<\/p>\n\n\n\n

    \n
  1. prof. dr. sc. Mile D\u017eelalija, <\/strong>Prirodoslovno-matemati\u010dki fakultet Sveu\u010dili\u0161ta u Splitu, predsjednik,<\/li>\n\n\n\n
  2. prof. dr. sc. Damir Sapunar, <\/strong>Medicinski fakultet Sveu\u010dili\u0161ta u Splitu, \u010dlan,<\/li>\n\n\n\n
  3. doc. dr. sc. Lucija Krce, <\/strong>Prirodoslovno-matemati\u010dki fakultet Sveu\u010dili\u0161ta u Splitu, \u010dlanica,<\/li>\n\n\n\n
  4. izv. prof. dr. sc. Larisa Zorani\u0107, <\/strong>Prirodoslovno matemati\u010dki fakultet Sveu\u010dili\u0161ta u Splitu, zamjenska \u010dlanica.<\/li>\n<\/ol>\n\n\n\n

    Mentorica:  izv. prof. dr. sc. Marija Ragu\u017e, <\/strong>Medicinski fakultet u Splitu.<\/p>\n\n\n\n

    Pozivaju se svi zainteresirani da prisustvuju obrani doktorskog rada.<\/p>\n\n\n\n

    Naslov:  <\/strong>Optimizacija elektroformacije divovskih unilamelarnih vezikula za razli\u010dite lipidne smjese uz naglasak na visoke koncentracije kolesterola<\/strong><\/p>\n\n\n\n

    SA\u017dETAK<\/strong>:
    Divovske unilamelarne vezikule (DUV) na\u0161iroko se koriste kao modeli stani\u010dnih membrana. Elektroformacija je naj\u010de\u0161\u0107e kori\u0161tena metoda za njihovu proizvodnju. Metoda ovisi o vi\u0161e parametara, tako da ponovljiva proizvodnja visokokvalitetnih DUV-ova mo\u017ee biti izazovna, osobito pri radu s lipidnim smjesama koje sadr\u017ee koncentracije kolesterola koje dose\u017eu i prema\u0161uju prag zasi\u0107enja membranskog dvosloja. Nakon analize postoje\u0107ih protokola elektroformacije, identificirali smo dva klju\u010dna problema za takve smjese. Prvi se odnosi na ponovljivost eksperimenata. Ve\u0107ina protokola koristi metodu nakapavanja koja proizvodi lipidne filmove nejednake debljine, \u0161to rezultira smanjenom ponovljivo\u0161\u0107u eksperimenata i u\u010dinkovito\u0161\u0107u stvaranja DUV-ova. Drugi je izlu\u010divanje kolesterola u obliku dehidriranih kristala tijekom su\u0161enja lipidnog filma, \u0161to dovodi do artefaktualnog smanjenja koncentracije kolesterola u DUV-ovima u usporedbi s po\u010detnom lipidnom smjesom. Kako bismo umanjili prvi problem, testirali smo elektroformaciju iz takvih smjesa uz zamjenu koraka nakapavanja tehnikom brzog razmazivanja. Optimizacija ovog procesa pobolj\u0161ala je veli\u010dinu i prinos DUV-ova, ali i dalje je preostalo pitanje izlu\u010divanja kolesterola. Potaknuti time, razvili smo novi protokol koji kombinira tehnike brze izmjene otapala, \u010di\u0161\u0107enja plazmom i brzog razmazivanja za proizvodnju DUV-ova elektroformacijom iz vla\u017enih lipidnih filmova. Smatramo da \u0107e nam ovaj novi protokol pomo\u0107i u daljnjem istra\u017eivanju svojstava membrana s vrlo visokim udjelom kolesterola, poput vlaknastih stanica le\u0107e oka.<\/p>\n\n\n\n

    Title:\u00a0<\/strong>Optimization of giant unilamellar vesicle electroformation for different lipid mixtures with a focus on high cholesterol concentrations<\/strong><\/p>\n\n\n\n

    ABSTRACT<\/strong>:<\/p>\n\n\n\n

    Giant unilamellar vesicles (GUVs) are widely used as artificial cell membrane models. Electroformation is the most commonly used method for their production. The method depends on multiple parameters, so reproducible production of high quality GUVs can be challenging, especially when working with lipid mixtures containing cholesterol concentrations reaching and surpassing the bilayer saturation threshold. After analyzing existing electroformation protocols, we identified two key issues for such mixtures. The first issue is related to experiment reproducibility. Most protocols use the drop-deposition method which produces lipid films of nonuniform thickness, resulting in lower experiment reproducibility and efficiency of GUV formation. The second is cholesterol demixing in the form of anhydrous crystals during lipid film drying, resulting in an artifactual decrease of cholesterol concentration in GUVs compared to the initial lipid mixture. To deal with the first problem, we first tested electroformation from such mixtures with replacement of the drop-deposition step by spin-coating. Optimizing this process improved the GUV size and yield, but the cholesterol demixing issue remained. To address this issue, we developed a new protocol which combines the rapid solvent exchange, plasma cleaning and spin-coating techniques to produce GUVs by electroformation from damp lipid films. We believe that this new improved electroformation protocol will allow us to successfully study models of eye lens fiber cell membranes with their very high Chol content.<\/p>\n","protected":false},"excerpt":{"rendered":"

    OBAVIJEST Javna obrana doktorskog rada studenta poslijediplomskog sveu\u010dili\u0161nog (doktorskog) studija BIOFIZIKA ZVONIMIRA BOBANA, mag. phys. pod naslovom \u201cOPTIMIZACIJA ELEKTROFORMACIJE DIVOVSKIH UNILAMELARNIH VEZIKULA ZA RAZLI\u010cITE LIPIDNE SMJESE UZ NAGLASAK NA VISOKE KONCENTRACIJE KOLESTEROLA\u201d odr\u017eat \u0107e se u \u010detvrtak, 27. travnja 2023., u 15:00 sati na Prirodoslovno-matemati\u010dkom fakultetu u Splitu (amfiteatar A1-2), pred \u010dlanovima Stru\u010dnog povjerenstva: Mentorica:  izv. prof. dr.<\/p>\n","protected":false},"author":1,"featured_media":701,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[26,16],"tags":[],"class_list":["post-352","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-defences-of-doctoral-theses","category-news-and-updates"],"acf":[],"_links":{"self":[{"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/posts\/352","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/comments?post=352"}],"version-history":[{"count":3,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/posts\/352\/revisions"}],"predecessor-version":[{"id":1212,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/posts\/352\/revisions\/1212"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/media\/701"}],"wp:attachment":[{"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/media?parent=352"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/categories?post=352"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/tags?post=352"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}