{"id":424,"date":"2020-07-27T14:43:00","date_gmt":"2020-07-27T12:43:00","guid":{"rendered":"https:\/\/biophysics.pmfst.unist.hr\/?p=424"},"modified":"2023-10-11T13:28:39","modified_gmt":"2023-10-11T11:28:39","slug":"javna-obrana-doktorskog-rada-lucije-krce","status":"publish","type":"post","link":"https:\/\/biophysics.pmfst.unist.hr\/en\/javna-obrana-doktorskog-rada-lucije-krce\/","title":{"rendered":"Javna obrana doktorskog rada Lucije Krce"},"content":{"rendered":"\n<p><strong>OBAVIJEST<\/strong><\/p>\n\n\n\n<p>Javna obrana doktorskog rada studentice poslijediplomskog sveu\u010dili\u0161nog studija BIOFIZIKA<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>LUCIJE KRCE<\/strong><\/h2>\n\n\n\n<p>pod naslovom<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>\u201cEksperimentalno istra\u017eivanje i modeliranje bakterijskog rasta i inaktivacije:<br><em>E. coli<\/em> izlo\u017eena laserski sintetiziranim srebrnim nano\u010desticama\u201d<\/strong><\/h2>\n\n\n\n<p>odr\u017eat \u0107e se u <strong>petak, 3. srpnja 2020., u 13:00 sati<\/strong> na Prirodoslovno-matemati\u010dkom fakultetu u Splitu (amfiteatar A0-2), pred \u010dlanovima Stru\u010dnog povjerenstva:<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>prof. dr. sc. Ante Bilu\u0161i\u0107 (Prirodoslovno-matemati\u010dki fakultet, Split) \u2013 predsjednik,<\/li>\n\n\n\n<li>dr. sc. Nik\u0161a Krstulovi\u0107 (Institut za fiziku, Zagreb) \u2013 \u010dlan,<\/li>\n\n\n\n<li>izv. prof. dr. sc. Marija Ragu\u017e (Medicinski fakultet, Split) \u2013 \u010dlanica.<\/li>\n<\/ol>\n\n\n\n<p><strong>Mentor<\/strong>: prof. dr. sc. Ivica Aviani (Prirodoslovno-matemati\u010dki fakultet, Split).<\/p>\n\n\n\n<p>Naslov:<br><strong>Eksperimentalno istra\u017eivanje i modeliranje bakterijskog rasta i inaktivacije:<br><em>E. coli<\/em> izlo\u017eena laserski sintetiziranim srebrnim nano\u010desticama<\/strong><\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Sa\u017eetak:<\/h3>\n\n\n\n<p>Cilj ove teze je razjasniti na\u010din antibakterijskog djelovanja koloidnih nano\u010destica srebra protiv <em>Escherichia coli<\/em> kao modelnog organizma. Istra\u017eivanje baktericidnog mehanizma srebrnih nano\u010destica je izvr\u0161eno kroz dva pristupa: eksperiment i modeliranje rasta tretiranih bakterijskih stanica. Srebrne nano\u010destice su sintetizirane laserskom ablacijom u vodi, a dobiveni koloid je temeljito karakteriziran. Uveden je model stani\u010dnog rasta temeljen na interakciji kako bi se razumio u\u010dinak nano\u010destica na dinamiku rasta bakterijskih stanica. Model obja\u0161njava rast netretiranih i tretiranih stanica <em>Escherichia coli<\/em> u teku\u0107oj kulturi. Glavne mjerne tehnike kori\u0161tene za eksperimentalno prou\u010davanje baktericidnog mehanizma nano\u010destica su fluorescentna spektroskopija te mikroskopija i spektroskopija atomskom silom. Istra\u017eivanje pokazuje da na\u010din antibakterijskog djelovanja srebrnih nano\u010destica na bakterijske stanice uklju\u010duje prodor unutar stanice\/membrane, permeabilizaciju membrane, smanjenje vremenski ovisnog Youngovog modula te mogu\u0107e istjecanje unutarstani\u010dnog materijala dok je razina reaktivnih kisikovih vrsta u smrtonosno tretiranim stanicama zanemariva.<\/p>\n\n\n\n<p>Title:<br><strong>Experimental investigation and modelling of bacterial growth and inactivation:<br><em>E. coli<\/em> exposed to laser synthesized silver nanoparticles<br><\/strong><\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Abstract:<\/h3>\n\n\n\n<p>The aim of this thesis is to elaborate on the mode of antibacterial action by colloidal silver nanoparticles against <em>Escherichia coli<\/em> as a model organism. Two approaches were used to investigate the bactericidal mechanism of silver nanoparticles (AgNPs): experimental probing and modelling of the treated bacterial cell growth. AgNPs were synthesized by laser ablation in water and the obtained colloid was thoroughly characterized. An upgradable interaction-based growth model was developed to understand the effect of AgNPs on bacterial cell dynamics. The model explains untreated and AgNP-treated <em>Escherichia coli<\/em> growth in batch culture. Subsequently, fluorescence measurements and atomic force microscopy and spectroscopy were used to experimentally study the AgNP bactericidal mechanism. This study shows that the mode of antibacterial action on silver nanoparticles includes penetration of AgNPs inside the cell\/membrane, membrane permeabilization, reduction of the cell\u2019s Young modulus in time and possible cell leakage while undistinguished reactive oxygen species level was detected inside lethally treated cells.<\/p>\n\n\n\n<p><a href=\"https:\/\/biophysics.pmfst.unist.hr\/wp-content\/uploads\/2023\/10\/Obavijest_o_javnoj_obrani_doktorskog_rada_LKrce.pdf\" data-type=\"link\" data-id=\"https:\/\/biophysics.pmfst.unist.hr\/wp-content\/uploads\/2023\/10\/Obavijest_o_javnoj_obrani_doktorskog_rada_LKrce.pdf\">Obavijest \u2013 javna obrana doktorskog rada Lucije Krce<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>OBAVIJEST Javna obrana doktorskog rada studentice poslijediplomskog sveu\u010dili\u0161nog studija BIOFIZIKA LUCIJE KRCE pod naslovom \u201cEksperimentalno istra\u017eivanje i modeliranje bakterijskog rasta i inaktivacije:E. coli izlo\u017eena laserski sintetiziranim srebrnim nano\u010desticama\u201d odr\u017eat \u0107e se u petak, 3. srpnja 2020., u 13:00 sati na Prirodoslovno-matemati\u010dkom fakultetu u Splitu (amfiteatar A0-2), pred \u010dlanovima Stru\u010dnog povjerenstva: Mentor: prof. dr. sc. Ivica<\/p>\n","protected":false},"author":1,"featured_media":701,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[26,16],"tags":[],"class_list":["post-424","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-defences-of-doctoral-theses","category-news-and-updates"],"acf":[],"_links":{"self":[{"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/posts\/424","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/comments?post=424"}],"version-history":[{"count":2,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/posts\/424\/revisions"}],"predecessor-version":[{"id":719,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/posts\/424\/revisions\/719"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/media\/701"}],"wp:attachment":[{"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/media?parent=424"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/categories?post=424"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/tags?post=424"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}