{"id":55,"date":"2023-05-16T13:15:00","date_gmt":"2023-05-16T11:15:00","guid":{"rendered":"https:\/\/biophysics.pmfst.unist.hr\/?p=55"},"modified":"2023-10-11T13:00:37","modified_gmt":"2023-10-11T11:00:37","slug":"javna-obrana-teme-doktorskog-rada-luke-gujinovica-mag-phys","status":"publish","type":"post","link":"https:\/\/biophysics.pmfst.unist.hr\/en\/javna-obrana-teme-doktorskog-rada-luke-gujinovica-mag-phys\/","title":{"rendered":"Javna obrana teme doktorskog rada Luke Gujinovi\u0107a, mag. phys."},"content":{"rendered":"\n

OBAVIJEST<\/strong><\/p>\n\n\n\n

Javna obrana teme<\/strong> doktorskog rada studenta poslijediplomskog sveu\u010dili\u0161nog (doktorskog) studija BIOFIZIKA<\/p>\n\n\n\n

LUKE GUJINOVI\u0106A, mag. phys.<\/strong><\/h2>\n\n\n\n

pod naslovom<\/p>\n\n\n\n

\u201cDinamika adhezije i raspr\u0161enja morske bakterije na \u010dvrstom supstratu\u201d<\/strong><\/h2>\n\n\n\n

odr\u017eat \u0107e se u srijedu, 24. svibnja 2023., u 12.00 sati<\/strong> na Prirodoslovno-matemati\u010dkom fakultetu Sveu\u010dili\u0161ta u Splitu (dvorana B3-48), pred \u010dlanovima Stru\u010dnog povjerenstva:<\/p>\n\n\n\n

    \n
  1. prof. dr. sc. Ante Bilu\u0161i\u0107<\/strong>, Prirodoslovno-matemati\u010dki fakultet Sveu\u010dili\u0161ta u Splitu \u2013 predsjednik,<\/li>\n\n\n\n
  2. dr. sc. Nelly Henry<\/strong>, Research Director, French National Centre for Scientific Research u Parizu \u2013 \u010dlanica,<\/li>\n\n\n\n
  3. izv. prof. dr. sc. Marija Ragu\u017e<\/strong>, Medicinski fakultet u Splitu \u2013 \u010dlanica.<\/li>\n<\/ol>\n\n\n\n

    Mentor i komentor<\/strong>:<\/p>\n\n\n\n

    prof. dr. sc. Dra\u017een Zanchi<\/strong> \u2013 MSC laboratory, UFR de Physique, Faculte des Sciences, Universite de Paris Cite
    doc. dr. sc. Ivica \u0160amani\u0107<\/strong> \u2013 Prirodoslovno-matemati\u010dki fakultet Sveu\u010dili\u0161ta u Splitu.<\/p>\n\n\n\n

    Pozivaju se svi zainteresirani da prisustvuju javnoj obrani.<\/p>\n\n\n\n

    Naslov:<\/strong><\/p>\n\n\n\n

    Dinamika adhezije i raspr\u0161enja morske bakterije na \u010dvrstom supstratu<\/strong><\/p>\n\n\n\n

    Sa\u017eetak<\/strong>:<\/p>\n\n\n\n

    Fokus moje doktorske disertacije je dinamika adhezije morskih bakterija na \u010dvrste povr\u0161ine<\/em>, sa Vibrio gigantis<\/em>-om, kao modelnom vrstom. Kao autohtona morska bakterija koja i sudjeluje, uz nekoliko drugih bakterijskih vrsta, u prvim fazama formacije biofilma, \u010dini svestran model za izu\u010davanje bioobra\u0161taja. Po\u010detna istra\u017eivanja podrazumijevaju izolaciju i optimiziranje postupka uzgoja vrste V. gigantis te potvr\u0111ivanje tendencije adhezije na \u010dvrstu podlogu. Nakon toga, na temelju metagenomskih podataka dobivenih metodom NGS (sekvenciranje idu\u0107e generacije), odredit \u0107e se relativna abundancija i prisutnost vrste V. gigantis<\/em> u raznim fazama kompleksnog morskog biofilma, kao i sastav klju\u010dnih gena za bakterijsku adheziju, kolektivno osje\u0107anje (engl. quorum sensing<\/em>) i pokretljivost stanice funkcionalnom predikcijom analizom PICRUSt (filogenetsko ispitivanje zajednica rekonstrukcijom neopa\u017eenih stanja). Zatim \u0107e se osmisliti eksperiment u bio-reaktoru kako bi se kvantificirala dinamika adhezije. Uz pomo\u0107 svjetlosne mikroskopije, izra\u010dunat \u0107e se pokrivenost povr\u0161ine i distribucija veli\u010dine kolonija u razli\u010ditim vremenima. Da bi se detaljnije de\u0161ifrirala adhezijska dinamika, dizajnirat \u0107e se eksperiment za pra\u0107enje adhezijske dinamike u stvarnom vremenu, u malom ba\u010dvastom spremniku i mikrokanali\u0107u, od kojih \u0107e oba biti izvedena na invertnom mikroskopu visoke rezolucije i uz osiguranu izmjenu bujona (medija). Nadalje, evolucija kolonija nastalih od manjeg broja razdvojenih stanica u \u010distom bujonu \u0107e se kvantificirati i zatim analizirati te usporediti sa standardnim modelima rasta. Da bi se shvatila kolektivna pojava prisutna u kolonizaciji, provest \u0107e se analiza dodatnog istra\u017eivanja evolucije i konzistencije kolonija u PDMS (Polydimethylsiloxane) mikrofluidnom kanali\u0107u U-oblika. Rezultati \u0107e se diskutirati prvo na nivou dinamike adhezije pojedina\u010dne bakterije, a zatim i kao kolektivni efekt.<\/p>\n\n\n\n

    Title:<\/strong><\/p>\n\n\n\n

    Adhesion and dispersion dynamics of marine bacteria on a solid substrate<\/strong><\/p>\n\n\n\n

    Abstract<\/strong>:<\/p>\n\n\n\n

    The focus of my doctoral thesis is the adhesion dynamics of marine bacteria to solid surfaces, <\/em>with Vibrio gigantis<\/em> as a model species. As an autochthonous marine bacterium, participating, among several other bacterial species, in the first stages of biofilm formation, it constitutes a versatile model for studying biofouling. Initial research includes isolation and optimization of V.  gigantis <\/em>species cultivation and confirmation of the tendency of adhesion to a solid substrate. After that, based on the metagenomic data obtained by the NGS (next generation sequencing) method, the relative abundance and presence of V. gigantis<\/em> species in various phases of the complex marine biofilm will be determined, as well as the composition of crucial genes for bacterial adhesion, quorum sensing, and cell motility by PICRUSt analysis (phylogenetic community examination by reconstruction of unobserved states) functional prediction. An experiment in a bio-reactor will be designed for the adhesion dynamics quantification. Using light microscopy, the surface coverage and the size distribution of colonies at different times will be monitored. In order to decipher the adhesion dynamics in more detail, we will design experiments for real-time tracking of adhesion dynamics in a smaller barrel-like container and microchannel, both carried out using high-resolution inverted microscopy with ensured broth (medium) exchange. Furthermore, the evolution of colonies originating from a small number of separated cells in a clean broth will be quantified, and subsequently analysed and compared to standard growth models. To decipher the collective phenomena involved in colonization, additional examination analysis of colony evolution and consistency in a PDMS (Polydimethylsiloxane) U-shaped microfluidic channel will be carried out. The results will be discussed first at the level of single bacterium adhesion dynamics and then as a collective effect.<\/p>\n","protected":false},"excerpt":{"rendered":"

    OBAVIJEST Javna obrana teme doktorskog rada studenta poslijediplomskog sveu\u010dili\u0161nog (doktorskog) studija BIOFIZIKA LUKE GUJINOVI\u0106A, mag. phys. pod naslovom \u201cDinamika adhezije i raspr\u0161enja morske bakterije na \u010dvrstom supstratu\u201d odr\u017eat \u0107e se u srijedu, 24. svibnja 2023., u 12.00 sati na Prirodoslovno-matemati\u010dkom fakultetu Sveu\u010dili\u0161ta u Splitu (dvorana B3-48), pred \u010dlanovima Stru\u010dnog povjerenstva: Mentor i komentor: prof. dr.<\/p>\n","protected":false},"author":1,"featured_media":690,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[24,16],"tags":[],"class_list":["post-55","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-defense-of-topics-of-doctoral-theses","category-news-and-updates"],"acf":[],"_links":{"self":[{"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/posts\/55","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/comments?post=55"}],"version-history":[{"count":3,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/posts\/55\/revisions"}],"predecessor-version":[{"id":667,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/posts\/55\/revisions\/667"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/media\/690"}],"wp:attachment":[{"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/media?parent=55"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/categories?post=55"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biophysics.pmfst.unist.hr\/en\/wp-json\/wp\/v2\/tags?post=55"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}